FIG. 2.

Growth curves of E. coli ATCC 25922 (A), P. aeruginosa ATCC 27853 (B), and S. aureus ATCC 29213 (C) demonstrating that a biofilm of a specific size can be produced over a specific period of growth on the CBD. The number of bacteria per peg was determined by breaking pegs from the lid at appropriate times and determining the bacterial number as described in Materials and Methods.