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. 2021 Oct 6;598(7879):86–102. doi: 10.1038/s41586-021-03950-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a, Confusion matrices between mouse L5 ET RNA-seq clusters and SCF, MERFISH, human or marmoset clusters, with the fraction of cells in each of the other datasets mapped to mouse transcriptomic clusters. b, Distribution of MERFISH L5 ET cells in upper or lower L5. L5_ET_4 cells are not found in this section. c, Genome browser view of gene markers for the MY-projecting (Slco2a1) and non-MY-projecting (Npnt) L5 ET neurons. d, Left, integration UMAP panels between L5 ET Epi-retro-seq clusters and consensus transcriptomic clusters using the snRNA 10x v3 B dataset with the largest number of L5 ET cells (more than 4,000). Right, confusion matrices (normalized by columns). e, Local dendritic and axonal morphologies (left) and brain-wide axon projections (right) of fully reconstructed MY-projecting and non-MY-projecting L5 ET neurons. Black, apical dendrites; blue, basal dendrites; red, axons. Numbers are cell IDs. f, Characterization of two L5 ET driver lines. For each line, the first panel shows tdTomato reporter expression from a Slco2a1-P2A-Cre;Ai14 or Npnt-P2A-FlpO;Ai65F mouse. The second panel shows a whole-brain view of the projection pattern from a Slco2a1-P2A-Cre mouse injected with AAV-pCAG-FLEX-EGFP-WPRE or a Npnt-P2A-FlpO mouse injected with AAV-pCAG-fDIO-mNeonGreen-WPRE (MOp (purple), TH (blue) and MY (yellow); injection sites indicated by the dashed circles). Last two panels show GFP- or mNeonGreen-labelled neurons at the injection site (MOp) and axon fibres in contralateral MY (seen only in Slco2a1 but not Npnt).