Extended Data Fig. 9. Retro-seq characterization of cortical IT neurons across all layers from MOs, MOp, SSp and SSs.

Transcriptomes of retrogradely labeled neurons were obtained by single cell or nucleus RNA-sequencing and then mapped to our transcriptomic taxonomy³¹ to identify the transcriptomic type (shown as clusters at the top and bottom of the dot plot) of each neuron. Cells are grouped by their source region. Within each source region, cells labeled from different projection targets (Injection Target region of interest, ROI) are compared, and found to be assigned to a similar subset of transcriptomic types without major distinction between ROIs. cMOs or cMOp denotes contralateral MOs or MOp, respectively.