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. 2021 Oct 6;598(7879):174–181. doi: 10.1038/s41586-021-03941-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 11 — a, Transcriptomic taxonomy of the entire mouse isocortex and hippocampal formation³¹ reveals a distinct branch of Car3 subclass (dashed box). Bar graph shows the distribution of Retro-seq cells within the Car3 subclass b, Enlarged view of the taxonomy part within the dashed box in a shows the distribution of Retro-seq cells in the 3 clusters of the Car3 subclass. c, Neurons from claustrum (CLA) are also entirely mapped to the Car3 subclass. We combined all CLA and cortical Car3 neuron SMART-Seq transcriptomes and re-clustered them to see if more refined cluster segregation could be obtained, resulting in 8 clusters. Dot plot shows the number of cells from each cortical region or CLA contributing to each cluster. d, Remapping of Retro-seq cells from CLA and several cortical regions (TEa-PERI-ECT and SSs) to the 8 new transcriptomic clusters. e, f, Marker gene expression for different clusters is similar between (e) cells contributing to the clustering shown in c and (f) Retro-seq cells shown in d.