Extended Data Fig. 18. related to Fig. 4. BARseq projection mapping in MOp compared to other data modalities.

a, Violin plots of the distribution of neurons with the indicated projections across cortical layers. Red crosses and green squares indicate means and median values, respectively. b, Mean normalized projection strengths of neurons in the indicated sublayers. Projection strengths were normalized so that the standard deviation for a projection across all neurons was 0. Black diamonds indicate p < 0.05 for the distribution of the projection strengths in the two adjacent sublayers using two-tailed rank sum test after Holms-Bonferroni correction. p values before correction are shown in Source Data Fig. 2. c, d, Projection patterns from single-cell tracing (c) and BARseq (d) shown at a common resolution achieved by both datasets. e, f, t-SNE plots of combined BARseq and single-cell tracing datasets color-coded by combined cluster classes (e) or by datasets (f). g, Number of neurons in each combined clusters that belong to each dataset. h, i, MetaNeighbor analysis of subgroups of all neurons (h) or IT neurons (i) identified by BARseq and tracing. Higher scores reflect stronger similarity between clusters. j, k, Similarity of sub-sampled BARseq and tracing neurons of the indicated clusters to their cluster centroids (j) or to the centroids of the matching clusters in the other dataset (k).