Fig. 1. Measuring RNA with multiple platforms.

RNA is measured using gene-tagging techniques such as the 10x Chromium scRNA-seq protocol, isoform sequencing techniques such as SMART-seq, and spatial RNA-capture techniques such as MERFISH. High-cell-throughput gene tagging enables cell-type identification with marker genes and deep full-length isoform sequencing enables cell-type marker refinement at the isoform level. Spatial RNA capture coupled with gene tagging and isoform sequencing enables spatial resolution of cell-type-specific isoform markers. The multi-method procedure for sampling RNA enables inference of spatially resolved cell-type-specific isoforms that no single technique could achieve independently⁵¹.