Fig. 3.

Influence of CoA and its acyl derivatives on pantothenate kinases. Multi-copy expression plasmids pSBS5 (MET25-CAB1; wild-type; a–d), pJO73 (MET25-PANK3; murine wild-type; e), and pEB27 (MET25-CAB1 W331R; hyperactive yeast allele; f) were transformed into strain JS91.14–24 (cab1^ts). To remove contaminating soluble yeast metabolites, crude extracts of transformants were partially purified by PanK binding to DEAE sepharose, elution, and subsequent dialysis. Varying concentrations of CoA (a), acetyl-CoA (b, e, f), malonyl-CoA (c), and palmitoyl-CoA (d) as indicated were added to PanK assay mixtures. Relative PanK activities (%) refer to enzyme activities in the absence of CoA inhibitors (100%). Standard deviations for at least four independent assays per inhibitor concentration are shown