Fig. 8.

Influence of media supplementation on biosynthesis of CoA nucleotides. CoA and acetyl-CoA were simultaneously measured by enzymatic analysis in protein-free cell extracts of reference strain JS91.15–23 and transformant MGY22. Additional TPI1-dependent genes introduced into MGY22 are indicated by + . Transformants were cultivated in synthetic complete medium (SCD; 0.4 mg/l Ca-pantothenate; 0.84 μM) or rich medium (YPD), both containing 2% glucose as a carbon source. Media were supplemented to final concentrations of 200 mg/l β-alanine (βA; 2.25 mM) and 20 mg/l Ca-pantothenate (Pan, 50-fold compared with standard-SCD, 42 μM), respectively. Standard deviations are indicated by error bars