Table 2.
Plasmids for targeting of expression cassettes at selected genomic sites
| Plasmid | Genetic markers |
|---|---|
| pLEUTEX-CAB1 | ZPS1-5´-flank::TPI1PRO-HA3-CAB1-CYC1TER::ZPS1-3´-flank |
| pLEUTEX-CAB1 W331R | ZPS1-5´-flank::TPI1PRO-HA3-CAB1W331R-CYC1TER::ZPS1-3´-flank |
| pLEUTEX-CAB2 | HIP1-5´-flank::TPI1PRO-HA3-CAB2-CYC1TER::HIP1-3´-flank |
| pLEUTEX-CAB3 | ENO1-5´-flank::TPI1PRO-HA3-CAB3-CYC1TER::ENO1-3´-flank |
| pLEUTEX-CAB4 | MRH1-5´-flank::TPI1PRO-HA3-CAB4-CYC1TER::MRH1-3´-flank |
| pLEUTEX-CAB5 | ADH3-5´-flank::TPI1PRO-HA3-CAB5-CYC1TER::ADH3-3´-flank |
| pLEUTEX-HAL3 | CUP9-5´-flank::TPI1PRO-HA3-HAL3aa260-495-CYC1TER::CUP9-3´-flank |
| pLEUTEX-FEN2 | GAT1-5´-flank::TPI1PRO-HA3-FEN2-CYC1TER::GAT1-3´-flank |
In addition, all plasmids carry LEU2 as a selection marker together with loxP sequences (loxP::LEU2::loxP) between CYC1TER und the 3´-flank