Figure 1.

TREM2 agonism induces PLC activity in iPSC- derived macrophages. (A) Representative Western blot showing phosphorylation of SYK after TREM2 stimulation for 5 min using a TREM2-specific antibody (AF1828, R&D Systems) in Parent and TREM2 Ko cells. Full immunoblot images are presented in Supplementary Fig. 6. (B) Ca²⁺ flux induced by TREM2 ligation in Parent cells is absent in TREM2 Ko cells. n = 4. (C) SYK inhibitor BIIB-057 (SYKi) reduces TREM2 antibody-evoked Ca²⁺ signal in a dose-dependent manner in Parent cells. n = 3. (D) TREM2-induced IP1 accumulation determined by HTRF assay is prevented in TREM2 Ko cells. n = 3, data shown represent mean ± SEM, (B, D) two-way ANOVA followed by Bonferroni’s multiple comparison test, (C) one-way ANOVA followed by Bonferroni’s multiple comparison test, *p < 0.05, **p < 0.01, ***p < 0.001.