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. 2021 Oct 6;12(10):911. doi: 10.1038/s41419-021-04213-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A, B RT-PCR (A) and Western blot analysis (B) were used to detect the expression of ECHS1 in CRC cell lines. C Western blot analysis showed that ECHS1-knockout and -overexpression cells were constructed. D Left panel: heatmap of the LC–MS assay for SW480sgECHS1 and SW480sgNC cells. Right panel: heatmap of the LC–MS assay for HCT116-LV-ECHS1 and HCT116-LV-Ctrl cells. E Representative figures and data of the Transwell assay for SW480sgECHS1, SW480sgNC, and HCT116-LV-ECHS1 and HCT116-LV-Ctrl cells. Bars in the right panel represent the number of migrated and invaded cells. The triple asterisk (***) indicates P < 0.001. F Upper panel: pi chart of lipids associated with the elevation of endogenous ECHS1. Bars in the right panel represent the lipids associated with phospholipid metabolism. Lower panel: pi chart of lipids associated with the reduction in endogenous ECHS1. Bars in the right panel represent the lipids associated with phospholipid metabolism. G Schematic diagram of UGCG regulating ceramide glycosylation. H Western blot analysis showed the expression of UGCG when endogenous ECHS1 changed in CRC cells. I Histogram of ELISA results showing the expression of endogenous ceramide when ECHS1 was overexpressed or knocked out in CRC cells.