Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 6;12(10):911. doi: 10.1038/s41419-021-04213-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — A Western blot analysis of the expression of PI3K/Akt/mTOR pathway members (PI3K, Akt, phosphorylated AKT at Ser473, and phosphorylated mTOR) in ECHS1-overexpressing or ECHS1-knockout CRC cells. B Western blot analysis of the expression of PI3K/Akt/mTOR pathway members in ECHS1-overexpressing cells with the application of the inhibitor of UGCG eliglustat (left panel) or in ECHS1-knockout cells with the addition of C2 ceramide (right panel). C Western blot analysis of the expression of autophagy-related proteins (ATG5, Beclin1, and LC3II/LC3I). D Western blot analysis of phosphorylated mTOR, Beclin1, BCL2, and caspase3 in CRC cells with or without ECHS1 overexpression and Eliglustat (100 nmol). E Flow cytometry analysis of intracellular ROS content. Bars in the right panel represent the average fluorescence intensity. F IF assay analysis of JC-10 aggregates (red) and monomers (green) in the mitochondrial matrix with or without starvation (2 h) and Eliglustat (100 nmol). Bars of the right panel represent the ratio of the average fluorescence intensity of monomers (green) and aggregates (red). G Electron microscopy results show mitochondrial morphology (yellow arrows) and autophagosomes (red arrows) in ECHS1-knockout SW480 cells. H Histogram of flow cytometry assay showing the ratio of the average fluorescence intensity of apoptotic cells with or without Oxaliplatin (20 µg/µl) and Eliglustat (100 nmol).