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. 2021 Oct 6;11:19851. doi: 10.1038/s41598-021-98706-1

Figure 5.

Figure 5

VLPs prepared in NaNO3-Tris buffer were stable in the presence of serum. Size and PDI of particles were evaluated for VLP suspension in different serum concentrations (10%, 30%, and 50%) after 1-h incubation at 4 °C. (a,b) 100 mM NaNO3 (pH: 7), (c,d) 100 mM NaNO3 (pH: 8), (e,f) 100 mM NaNO3-Tris (pH: 8). Results are shown as median with range, p < 0.02, p < 0.001, p < 0.002. (g) Gel electrophoresis for RT-PCR fragments of shRNAs extracted from VLP samples that were incubated at 37 °C for 0 h, 2 h, 4 h, 6 h, 8 h, 10 h, 12 h, 24 h, and 48 h time points in NaNO3-Tris (pH: 8) and 50% of Serum.