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. 2021 Oct 6;11:19872. doi: 10.1038/s41598-021-97726-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Relative Cohen’s d (Supplementary Information) of drug administration calibrated against oxygen-air transition during initial gas challenge in Dataset A. In both constrained and unconstrained [Hb]^MSOT and [HbO₂]^MSOT images, there is a large region of anomalous relative effect (magenta arrows), likely due to changes in measured [Hb_tot]^MSOT over the course of the experiment. This large region is absent in the SO₂^MSOT analyses due to the ratiometric calculation. More localized artifacts such as those highlighted in the unconstrained analysis (white arrows) are due to modelling or reconstruction inaccuracies, and so are not compensated by calculating SO₂^MSOT. Figure created using a combination of MATLAB and Microsoft PowerPoint.