Table 1.
PCR primers used in the study.
| Target gene | Purpose | Name | Sequence of oligonucleotide (5’–3’) |
|---|---|---|---|
| NDV | qRT-PCR | NDV F | TGCAGCAATGGTACTCCGTT |
| NDV R | CCTTTGCTACCGTGACCCAT | ||
| chIFN-α | qRT-PCR | qIFN-α F | AACCACCCACGACATCCTTC |
| qIFN-α R | TGTCGCTGCTGTCCAAGC | ||
| chIFN-β | qRT-PCR | qIFN-β F | CCTCAACCAGATCCAGCATT |
| qIFN-β R | GGATGAGGCTGTGAGAGGAG | ||
| chIFN-λ | qRT-PCR | qIFN-λ F | CCTGGCCTTCCTTACCCAAG |
| qIFN-λ R | CTCAGTTTCCCAGAGGGCTG | ||
| chIRF-7 | qRT-PCR | qIRF-7 F | GCCTGAAGAAGTGCAAGGTC |
| qIRF-7 R | CTCTGTGCAAAACACCCTGA | ||
| chPKR | qRT-PCR | qPKR F | TGCTTGACTGGAAAGGCTACT |
| qPKR R | TCAGTCAAGAATAAACCATGTGTG | ||
| chMx-1 | qRT-PCR | qMx-1 F | GTTTCGGACATGGGGAGTAA |
| qMx-1 R | GCATACGATTTCTTCAACTTTGG | ||
| IL-6 | qRT-PCR | qIL-6 F | GACGAGGAGAAATGCCTGAC |
| qIL-6 R | GACTTCAGATTGGCGAGGAG | ||
| IL-8 | qRT-PCR | qIL-8 F | GCTCTGTCGCAAGGTAGGAC |
| qIL-8 R | GGCCATAAGTGCCTTTACGA | ||
| chβ-actin | qRT-PCR | qβ-actin F | CAGACATCAGGGTGTGATGG |
| qβ-actin R | TCAGGGGCTACTCTCAGCTC | ||
| chDDX1 | qRT-PCR | qDDX1 F | CGACAAACTCTGGGAAAGGC |
| qDDX1 R | TTGTGTTCCTTGATTGCCCG | ||
| To obtain sequence | chDDX1 F | ATGGCGGCGTTCTCGGAAAT | |
| chDDX1 R | TCAGAATGTTCTGAACAGCT | ||
| Construction of chDDX1 | p3×FLAG-14-CMV Kpn I F | gatagatctgatatcggtaccATGGCGGCGTTCTCGGAA | |
| p3×FLAG-14-CMV BamH I R | tttgtagtcagcccgggatccGAATGTTCTGAACAGCTGGTTAGG | ||
| Construct truncated forms of chDDX1 | d SPRY U | AGTCACACCTCTAGTTGC | |
| d SPRY L | caactagaggtgtgactTTCAAATTTCCACCAAAAGATGG | ||
| d HELICc U | ACAATCTATTTTTGTCCTGCAG | ||
| d HELICc L | gacaaaaatagattgtAGGATGGGTCTGGCAATTTC | ||
| d 1-100 aa U | GGTACCGATATCAGATCTATC | ||
| d 1-100 aa L | gatatcggtaccatgGCTTTTGCAATTGGATC | ||
| d 1-250 aa U | GGTACCGATATCAGATCTATC | ||
| d 1-250 aa L | gatatcggtaccatgCCAAAAGATGGCTATATTGG | ||
| d 1-500 aa U | GGTACCGATATCAGATCTATC | ||
| d 1-500 aa L | gatatcggtaccatgATCAAGGAACACAAGATGG | ||
| d 251-500 aa U | TGGAAATTTGAAGTCTTCTTCAC | ||
| d 251-500 aa L | gaagacttcaaatttccaATCAAGGAACACAAGATG | ||
| d 501-740 aa U | TGCCCGAACAGTGTACTC | ||
| d 501-740 aa L | tacactgttcgggcaGGATCCCGGGCTGACTACAAAG |