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. 2021 Sep 23;12:757231. doi: 10.3389/fimmu.2021.757231

Figure 4.

Figure 4

CBL-/-CBL-B-/- mice have a reduced lifespan and severe inflammation. (A) Body weight comparison between 12-week-old WT and CBL-/-CBL-B-/- mice (n = 5). (B) Kaplan-Meier survival analysis of WT (n = 20) and CBL-/-CBL-B-/- (n = 20) mice. (C) Pathological analysis WT and CBL-/-CBL-B-/- mice. Shown are H-E staining and Masson staining of the liver. Infiltrating leukocytes and liver fibrosis are indicated by arrows. (D) Flow cytometric analysis of liver leukocyte subsets. Shown are the statistics of infiltrated cell subsets of the gated CD45+ cells in livers from WT and CBL-/-CBL-B-/- mice (n = 5). DC: CD11c+MHC-IIhi; CD103+ DC: CD11c+MHC-IIhiCD103+CD8α+; CD11b+ DC: CD11c+MHC-IIhiCD11b+; CD4+ T: TCRβ+CD4+; CD8+ T: TCRβ+CD8+; Ly6G+ Gr: CD11b+Ly6G+; F4/80+ MΦ: CD11b+F4/80+. (E) Cytokine IL-6 and TNF-α secretion in serum (n = 5-6). (F) qPCR analysis of cytokine IL-6 and TNF-α expression in the liver (n = 3). Data are mean ± SEM. of at least two independent experiments (C–F). N.S., not significant; *p < 0.05; **p < 0.01; ***p < 0.001.