Table 1.
Compound and protocol information for paracetamol UPLC–MS/MS analysis.
| Component name | Manufacturer | Code | Expected RT (min) | Expected m/z | Fragment m/z |
|---|---|---|---|---|---|
| 13C6—Paracetamol | Cambridge Isotope Laboratories Inc | CLM-10619-PK | 1.85 | 158 | 116 |
| Paracetamol | SIGMA | 103-90-2 | 1.85 | 152 | 110 |
| 65 | |||||
| Paracetamol glucuronide | SIGMA | 16110-10-4 | 1.36 | 328 | 110 |
| 152 | |||||
| Paracetamol glutathione | NovaChem | A161223 | 1.92 | 457 | 382 |
| 328 | |||||
| 152 | |||||
| Paracetamol sulphate | SIGMA | 32113-41-0 | 2.31 | 232 | 152 |
| 110 |
The compound being analysed (component name) and relevant manufacturer information is detailed along with column retention time (RT) and the mass to charge ratio (m/z) for the parent and fragment ions. The m/z transition: m/z 158 > 116, m/z 152 > 110, m/z 328 > 152, m/z 457 > 328 and m/z 232 > 152 were used for quantitative analysis of 13C6-paracetamol, paracetamol, paracetamol glucuronide, paracetamol glutathione, and paracetamol sulphate respectively, while other transitions were used as qualifiers.