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. 2021 Oct 6;11:19871. doi: 10.1038/s41598-021-99237-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of MPTP and L-DOPA treatment on total TH and phospho-TH at Ser-40 expression in the monkey heart. (A, B) Total TH/β-actin ratio (optical density, % vs control) in controls, MPTP-treated monkeys and in the MPTP + L-DOPA group. Total TH expression was significantly decreased in the LV in MPTP-treated monkeys and in the MPTP + L-DOPA group versus control group and in the MPTP + L-DOPA group versus MPTP-treated animals. (C) Strong correlation between the number of TH + neurons in the SNpc and total TH levels in the heart tissue (r² = 0.85, P = 0.0004). (D, E) P-TH at Ser-40/β-actin ratio (optical density, % vs control) in controls, MPTP-treated monkeys and in the MPTP + L-DOPA group. TH phosphorylation at Ser-40 was significantly increased in the RV with respect to the other two groups. Besides, in the LV, we found an important increase of phospho-TH at Ser-40 in both groups (MPTP or MPTP + L-DOPA treatment) with respect to controls. (F) Strong correlation between phospho-TH and TH+ neurons in the SNpc (r² = 0.77, P = 0.0019). Data were compared by one-way ANOVA (total TH: F_(2,9) = 2.063, P = 0.1831, RV; F_(2,9) = 17.87, P = 0.0007, LV. P-TH: F_(2,9) = 12.77, P = 0.0024, RV; F_(2,9) = 17.43, P = 0.0008, LV) followed by Newman–Keuls post-hoc test. Values are represented as mean ± standard deviation. **P < 0.01, ***P < 0.001 versus control group; ^#P < 0.05 versus MPTP-treated animals; ⁺⁺P < 0.01 versus MPTP + L-DOPA. TH tyrosine hydroxylase, MPTP 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, L-DOPA levodopa, P phospho. (n = 4 animals/group and 2 technical replicates/animal).