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. 2021 Oct 6;11:19836. doi: 10.1038/s41598-021-99338-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cell lineage analysis of photoconverted SoxE3:Dendra2 knock-in lampreys. (A) Photoconversion experiments were performed in the head region of SoxE3:Dendra2 knock-in lampreys at stage 21. Native green fluorescence, photoconverted red fluorescence (shown in magenta), and the merged image are shown in (A), (A’), and (A’’), respectively. The midbrain–hindbrain boundary (MHB) is indicated with dashed lines. Scale bar: 500 μm. (B) The same animal shown in (A) is raised to the stage 24 and reexamined. Some photoconverted cells are observed in the mandibular arch (ma), suggesting ventral migration of these NCC cells (arrows). Native green fluorescence, photoconverted red fluorescence (shown in magenta), and the merged image are shown in (B), (B’), and (B’’), respectively. The midbrain–hindbrain boundary (MHB) is indicated with dashed lines. Scale bar: 200 μm.