Table 1.
Structures and crosslinking efficiencies for electrophilic and photoaffinity probes used for proximity induced covalent modification of the Tt-preQ1-RS aptamer (compounds 1–10) or Bs-preQ1-RS aptamer (compounds 11–16). Error bars indicate standard deviation, n = 3. (Molar ratio between the aptamer and probe is 1:50).
| Compound ID | Compound structure | % Crosslink efficiency (PAGE) |
|---|---|---|
| 1 |  |
Not observed |
| 2 |  |
Not observed |
| 3 |  |
Not observed |
| 4 |  |
Not observed |
| 5 |  |
Not observed |
| 6 |  |
Not observed |
| 7 |  |
Not observed |
| 8 |  |
Not observed |
| 9 |  |
Not observed |
| 10 |  |
Not observed |
| 11 |  |
31.3 ± 2.9 |
| 12 |  |
49.2 ± 3.2 |
| 13 |  |
39.9 ± 5.3 |
| 14 |  |
Trace |
| 15 |  |
22.0 ± 2.2 |
| 16 |  |
19.4 ± 4.4 |
| 17 |  |
Not determined |