Skip to main content
. 2021 Sep 23;11:716921. doi: 10.3389/fonc.2021.716921

Figure 7.

Figure 7

HNRNPA2B1 is correlated with the EMT process in OSCC. (A) EMT scores in normal and OSCC tissues. (B, C) Gene set enrichment analyses show the upregulated genes for HNRNA2B1 high-expression patients compared to low-expression patients. (D) GSE data (GSE138206) and TCGA data show that HNRNPA2B1 is correlated with multiple EMT markers. (E–H) HNRNPA2B1 expression is negatively correlated with E-cadherin (R = -0.35, p < 0.05) expression and positively correlated with N-cadherin (R = 0.53, p < 0.05) and vimentin (R = 0.56, p < 0.05), but was not correlated with the LINE-1 mRNA expression (R = 0.08, p = 0.15). (I) LINE-1 mRNA expression was not correlated with the HNRNPA2B1 expression in OSCC cell lines. (J) Western blot analysis of EMT markers related with LINE-1/TGF-β1/Snail signaling pathway. Protein levels of N-cadherin, E-cadherin, TGF-β1, Snail, and LINE-1 ORF1 of CAL27 and SCC4 cells were detected by Western blot after HNRNPA2B1 was knocked down or overexpressed. (K) Relative enrichment of LINE-1 mRNA associated with HNRNPA2B1 protein was identified by RIP assays using anti-IgG and anti-HNRNPA2B1 antibodies. The IgG group was a negative control to preclude nonspecific binding. The Y-axis represents the percent of input for each IP sample according to the formula: %Input =1/10*2Ct [IP]-Ct [input]. *, **, *** and **** indicated p<0.05, p<0.01, p<0.001 and p<0.0001, respectively. “ns” indicates no significance.