Skip to main content
. 2021 Sep 23;8:718674. doi: 10.3389/fcvm.2021.718674

Figure 2.

Figure 2

Butyrate treatment improved oxidative stress and cell apoptosis after myocardial I/R, and vagotomy diminished this protective effect. (A) Myocardial MDA and (B) SOD activities. (C) Representative images of TUNEL staining. Myocardial tissues were stained and analyzed with TUNEL (green) and counterstained with DAPI (blue). (D) Quantitative analysis of TUNEL positive cells expressed as percentage of DAPI stained cells. N.D., no detectable. Compared group: I/R, butyrate, vagotomy. (E) Representative images of Western blot of Bax and Bcl-2 expression from sham, I/R, butyrate, and vagotomy groups. (F,G) Relative intensity of Bax/Bcl2 over GAPDH (% sham). ***p < 0.001 vs. sham group, ****p < 0.0001 vs. sham group; ##p < 0.01 vs. I/R group, ###p < 0.001 vs. I/R group, ####p < 0.0001 vs. I/R group; &p < 0.05, &&p < 0.01, &&&p < 0.001 vs. butyrate group. SOD, superoxide dismutase; MDA, malondialdehyde; DAPI, 4',6-diamidino-2-phenylindole; I/R, ischemia/reperfusion; Bcl-2, B-cell lymphoma-2; Bax, BCL2-Associated X; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.