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TFLS inhibited the proliferation of PCa cells. (A,B) PC3 and DU145 cells were treated with different concentrations of TFLS (0–240 μg/ml) for 24, 48 and 72 h, and cell viability was detected by the MTS assay. (C) The colony formation of PC3 and DU145 cells treated with TFLS for 8 days. (D) Quantification of the number of colonies of PCa cells. The number of colonies was expressed by mean ± SEM, compared with the control group: *p < 0.05; **p < 0.01.
