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. 2021 Sep 23;12:758219. doi: 10.3389/fphar.2021.758219

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Copyright © 2021 Chang, Zhu, Chen, Zhang, Liu, Li, Cheng, Su, Zhang, Lu and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TFLS induced apoptosis in PCa cells. PC3. (A) and DU145. (B) cells were treated with TFLS, the apoptotic cells were analyzed by Annexin V and PI double staining followed by flow cytometry analysis. The percentage of early apoptotic cells, late apoptotic cells and overall apoptotic cells were determined. Bar plot of overall apoptotic cells in PC3. (C) and DU145. (D) cells (Annexin V positive cells). (E) The expressions of apoptosis-related proteins in PC3 and DU145 cells were evaluated by Western blot. (F) Bar plots of the relative expressions of apoptosis-related proteins in PC3 and DU145 cells. Data are expressed as mean ± SEM. Compared with control group: *p < 0.05; **p < 0.01.