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. 2021 Sep 23;54:101328. doi: 10.1016/j.molmet.2021.101328

Figure 1.

Figure 1

Generation of smaller adipocytes in the iWAT of HFD-fed male mice. (A) Schematic diagram of the experimental protocol. (B) Representative images of hematoxylin and eosin (H&E)-stained paraffin sections of gWAT and iWAT obtained from KI/+ male mice fed either NC or HFD for 8 weeks. Mice were started on HFD at 9 weeks of age and euthanized at 17 weeks. Scale bar = 250 μm (n = 4/group). (C) Representative images of H&E-stained paraffin sections of gWAT and iWAT obtained from KI/+ female mice fed either NC or HFD for 8 weeks. Mice were started on HFD at 9 weeks of age and euthanized at 17 weeks. Scale bar = 250 μm (n = 3/group). (D) Graphical diagram for the generation of KI/td mice. (E–G) Representative graphs of recombination efficiency analyzed by immunohistochemistry (E) and flow cytometry analysis in both depots (F and G). Tamoxifen (TAM) was administered at the age of 7 weeks for five consecutive days. Mice were euthanized at 9 weeks of age; gWAT and iWAT were collected and subjected to immunohistochemistry and FACS analysis (n = 8/group). (H) Body weight (g) of male and female KI/td mice fed either NC or HFD for 8 weeks. Mice were started on HFD at 9 weeks of age and euthanized at 17 weeks (Male: NC, n = 8; HFD, n = 8; female: NC, n = 8; HFD, n = 8). (I and J) Weight of the WAT depots (gWAT and iWAT) as % BW in male (I) and female (J) KI/td mice fed either NC or HFD for 8 weeks (Male: NC, n = 8; HFD, n = 8; female: NC, n = 8; HFD, n = 8) (BW; body weight). (K and L) Total number of tdTomato+ APs (CD31−CD45−Sca1+ cells) determined by flow cytometry in male (K) and female (L) KI/td mice fed either NC or HFD for 8 weeks. (Male: NC, n = 8; HFD, n = 7; female: NC, n = 5; HFD, n = 6). Data represent mean ± SEM. Statistical analysis was performed using Student's t-test (∗p < 0.05, ∗∗p < 0.01).