Figure 6.

Gene expression analysis of FACS-sorted tdTomato⁺labeled and non-labeled APs. (A) Representative flow cytometry image showing the gating strategy for isolating tdTomato⁺ and tdTomato⁻ APs from KI/td male mice fed HFD for 8 weeks. Mice were started on HFD at 9 weeks of age and euthanized at 17 weeks. (B–G) Gene expression analysis of markers of adipocyte progenitors in FACS-isolated fractions of tdTomato⁺ and tdTomato⁻ APs from KI/td male mice fed HFD for 8 weeks (n = 3). Data represent mean ± SEM. Statistical analysis was performed using Student's t-test (∗p < 0.05, ∗∗p < 0.01).