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. 2021 Sep 23;12:721045. doi: 10.3389/fgene.2021.721045

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Copyright © 2021 Rubinstein, McLean, Lehman, Meudt, Schomberg, Krentz, Reichert, Meyer, Adams, Konsitzke and Shanmuganayagam.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TP53 LOF model and CNV analysis. (A) Schematic depicts Cas9 target sites in exon 2 and exon 7 of TP53. Red arrowheads: Cas9 cut sites, single-headed black arrows (f1, f2, r1, r2): primer annealing sites for competitive and dPCR (distinct primer sets, see Supplementary Table 2 for primer sequences), “p” indicates dPCR probe annealing site. (B) Left: Allele sequences from each pig are provided, along with the CNV analysis corresponding to those sequences. Right: CNV analysis revealed that while some pigs carried the excision, others carried alleles that could not be amplified.