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. 2021 Sep 3;17(14):3850–3861. doi: 10.7150/ijbs.64630

Figure 2.

Figure 2

Generation of MSLN- and CD19-CAR NK cells. A. Schematic representation of MSLN- and CD19-CAR molecules. B. Percentage of MSLN- and CD19-CAR transduced CAR NK cells detected by flow cytometry. GFP served as a marker of CAR expression. C. Western blot analysis of CAR expression in transduced MSLN- and CD19-CAR NK cells. A CD3ζ-specific antibody was used to detect endogenous and chimeric CD3ζ. D. Relative expressions of different CAR mRNAs normalized to GAPDH in CAR NK cells were detected by qPCR.