Figure 3.

MSLN-CAR NK cells showed strong antitumor activity against MSLN-positive gastric cancer cell lines in vitro. A. GL expression was detected in N87 GL, MKN-28 GL, AGS GL and Huh-7 GL cell lines by flow cytometry. GFP served as a marker of luciferase expression. B. The effector cells were co-cultured for 6 hrs with target cells (1×10⁴ ) at E:T ratios of 2:1, 4:1, 8:1 and 16:1 in a total volume of 100 µL. C. Detection of IFN-γ, GM-CSF, Granzyme B and perforin secretion by effector cells after coculture with target cells for 24 hrs at an E:T ratio of 2:1. Data reflected the mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001.