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. 2021 Sep 7;17(14):3875–3888. doi: 10.7150/ijbs.62517

Figure 2.

Figure 2

HPV 18 E6/E7 proteins promote cervical cancer proliferation by up-regulating MELK through regulating transcription factor E2F1. A. Effects of HPV18 E6/E7 knockdown on MELK expression. HeLa cells were treated with HPV18 E6/E7 specific shRNA for 48h, and levels of total or phosphorylated proteins were determined by Western blot. GAPDH was used as a loading control. B. mRNA expression for the indicated genes (MELK, HPV 18 E6, HPV 18 E7) was measured in HeLa cells 48 hr after shNC, HPV18 E6/E7 specific shRNA. * P <0.05, ** P <0.01, *** P <0.001 and **** P <0.0001 compared to the shNC group. C. HeLa cells were treated with E2F1 specific siRNA for 72h, and levels of total proteins were determined by Western blot. GAPDH was used as a loading control. D. mRNA expression for the indicated genes (MELK, E2F1) was measured in HeLa cells 72h after siNC, E2F1 specific siRNA. * P <0.05, ** P <0.01, *** P <0.001 and **** P <0.0001 compared to siNC group.