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. 2021 Sep 7;17(14):3875–3888. doi: 10.7150/ijbs.62517

Figure 5.

Figure 5

MELK regulates multiple G2/M phase essential proteins. A and B. After MELK kinase activity was inhibited by 5 µM MELK-8A, HeLa cells showed polyploidy. DMSO was used as a control. C and D. HeLa cells were treated with 5 µM MELK-8A for 48 h, and levels of total proteins were determined by Western blot. GAPDH was used as a loading control. Note that the protein abundance of P-PLK1Tyr217, Cyclin B1, P-CDK1Tyr15, BAX, and Cleaved-PARP1 increases, but MELK or Bcl-2 was reduced after use of MELK-8A.