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. 2021 Sep 21;17(14):3968–3980. doi: 10.7150/ijbs.58910

Figure 1.

Figure 1

lnc-MEG3 is upregulated significantly and lnc-MEG3 silencing alleviates DDP-induced inflammation and apoptosis. (A) lnc-MEG3 expressions were visualized by R package “pheatmap” based on the RNA sequence from GEO database. Relative mRNA levels of lnc-MEG3 in DDP-treated HK-2 cells (B), the kidneys of DDPIN mice (C) on RT-PCR analysis. (D) FISH assays of lnc-MEG3 location at the cytoplasm fractionation of proximal tubule epithelial cells. Relative mRNA levels of lnc-MEG3 silenced HK-2 cells on RT-PCR analysis (E). Flow cytometry (F) and data analysis (G) of apoptosis in DDPIN or/and lnc-MEG3 silenced HK-2 cells. (H) RT-PCR analysis of IL-1, IL-6, IL-10, IL-18, MCP-1 and TNF-α. *P < 0.05, **P < 0.01, ***P < 0.001 versus control, #P < 0.05, ##P < 0.01, ###P < 0.001 versus DDP-treated control (n=3).