Skip to main content
. 2021 Sep 7;17(14):3898–3910. doi: 10.7150/ijbs.65488

Figure 3.

Figure 3

ADAM9 promotes the plasminogen activator-based pathway in ESCC. (A) Gene ontology-based functional analysis of ADAM9-regulated genes using differentially expressed genes in the control and ADAM9 KO KYSE170 cells. (B) tPA activity measured in the two-day culture conditioned media. Two individual clones of ADAM9 KO (#17 and #29) KYSE170 cells were analyzed. (C) Western blot analysis of indicated proteins in ADAM9 KO KYSE170 cells transiently transfected with plasmids of vector (V), ADAM9 wild type (WT), and E348A mutant. (D - F) The measured tPA activity (D), cell morphology; scale bar, 200 μm (E), and migration distance (F) of ADAM9 KO cells transiently transfected with plasmids of ADAM9 WT and E348A mutant. Bar chart data are mean ± SD. Student's t test. *, P < 0.05. **, P < 0.01. (G) The correlation of ADAM9 and PLAT or SERPINE1 RNA expression in ESCC patients from TCGA dataset.