Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 23;11:713026. doi: 10.3389/fonc.2021.713026

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Topalov, Mayr, Scherer, Chelariu-Raicu, Beyer, Hester, Kraus, Zheng, Kaltofen, Kolben, Burges, Mahner, Trillsch, Jeschke and Czogalla

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Functional assays after NFAT5 silencing in UWB1.289 cells. (A) ACTBL2 expression in UWB1.290 cells after 48h siRNA (sequence 7) knockdown of NFAT5, proving its successful downregulation on mRNA level compared to the untreated control (p=0.008). (B) MTT assay results after siRNA knockdown of NFAT5, showing a significantly reduced cell viability (n=8, p=0.012). (C) BrdU assay results, displaying a significantly decreased cell proliferation after silencing of NFAT5 (n=15, p=0.001) (D–J) Wound healing assay results (D) after siRNA knockdown of NFAT5, showing a significantly reduced migration of UWB1.289 cells after 48h (H–J) compared to the untreated control (E–G; n=8, p=0.012) (10x magnification; scale bar=200µm).