FIGURE 6.

RH suppressed the activation of NF-κB and the phosphorylation of ERK and P38 induced by RANKL. (A) RH inhibited the NF-κB activity of RAW 264.7 cells with luciferase report construct at the different concentrations of RH as indicated. The cells were pretreated with varying densities of RH and stimulated with RANKL at 50 ng/mL for 6 hours. (B–C) Representative images of the expression level of IĸB-α and β-actin in Western blot assay and the quantification of the ratios of band intensity of IκB-α to β-actin. (B–F) Representative images of the expression phosphorylation level of ERK and P38 with or without the treatment of 50 μM RH, and the quantification of the fold change ratios of band intensity of p-ERK to ERK and p-P38 to P38 (n = 3). *p < 0.05, **p < 0.01 relative to RANKL-induced positive group.