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. 2021 Sep 6;13(10):e13790. doi: 10.15252/emmm.202013790

Figure 1. Direct molecular interaction between the P5 and AdipoR1.

Figure 1

  1. Domain architectures of APN and the GST‐fused 5‐mer peptides (GLYYF; GST‐P5, or NIPGL; GST‐P10).
  2. Domain architectures of AdipoR1 and Flag‐fused full‐length AdipoR1 (residue 1–375) or N‐terminal‐truncated AdipoR1 (residues 89–375). Each construct in the schematic diagram was used for the pulldown experiment.
  3. GST pulldown of transiently expressed AdipoR1 (residues 1–375) with immobilized GST‐P5 or GST‐P10 fusion proteins. Pulldown fractions were subjected to Western blotting using Flag and GST antibodies.
  4. GST pulldown of AdipoR1 (residues 1–375) or AdipoR1 (residues 89–375) with GST‐P5. The red or blue arrow indicates correct sized bands of AdipoR1 (residues 1–375) or AdipoR1 (residues 89–375), respectively.
  5. The position of P5 in APN. A stereo ribbon diagram of APN (PDB ID: 4DOU) is drawn, and P5 is shown in magenta sticks.

Source data are available online for this figure.