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. 2021 Sep 20;22(5):1328. doi: 10.3892/etm.2021.10763

Figure 1.

Figure 1

miR-515-5p overexpression inhibits the cell proliferation of MCF7 and ZR-75-30 cells. MCF7 and ZR-75-30 cells were transfected with miR-515-5p or NC mimics. (A) RT-qPCR was used to measure the expression of miR-515-5p in different human breast cancer cell lines MCF7, ZR-75-30, MDA-MB-231 and SKBr-3 and in the normal breast cell line MCF10A. (B) RT-qPCR was used to detect the expression of miR-515-5p in MCF7 and ZR-75-30 cells 48 h after transfection. (C) Cell Counting Kit-8 assay was performed on ZR-75-30 and MCF-7 cells to measure cell viability. (D) Colony formation assay was performed to measure cell proliferation. The data are presented as the mean ± SD. **P<0.01 vs. NC mimic and MCF10A. miR, miRNA; NC, negative control; RT-qPCR, reverse transcription quantitative PCR; OD, optical density.