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. 2021 Oct 2;17:641–657. doi: 10.2147/VHRM.S288090

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© 2021 Wang et al.

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Application of scRNA-seq computational approach. Preprocessing steps convert the raw reads to sparse expression matrix. Downstream data analysis includes clustering, differentially expressed gene calling, cell trajectory analysis, RNA velocity, cell–cell interactions, identify mutations, integration (Reprinted from Cell, 177(7), Stuart T, Butler A, Hoffman P, et al. Comprehensive Integration of Single-Cell Data. 1888-1902 e182, Copyright 2019, with permission from Elsevier)⁶⁴ and spatial genomics.