Skip to main content
. 2021 Oct 15;31(3):030706. doi: 10.11613/BM.2021.030706

Table 1. The effects of haemolysis on complete blood count.

Parameter Group 1
(N = 10) 		Group 2
(N = 17) 		Group 3
(N = 16) 		Group 4
(N = 7) 		Group 5
(N = 9) 		APS, %
Haemolysis 0 1 0 2 0 3 0 4 0 6 Bias CVA CVG RCV
WBC
(x109 /L)		 8.01
(5.78-10.65)		 7.80
(6.09-11.35)		 8.01
(6.53-9.11)		 7.87
(6.47-9.59)		 6.90
(5.15-9.61)		 7.05
(4.98-10.13)		 8.01
(6.15-10.80)		 7.98
(6.42-10.80)		 6.16
(5.20-8.92)		 6.67
(5.30-8.96)
Bias
(95%CI)		 2.73 (-1.04 to 6.51) 2.04 (-0.09 to 4.17) 2.52 (0.38 to 4.66) 2.52 (-1.99 to 7.03) 4.00(1.07 to 6.93) ± 4.9 1.82 10.80 30.6
Neu
(x109/L)		 4.71
(3.10-7.61)		 4.64
(3.33-8.12)		 4.71
(2.83-5.55)		 4.77
(2.94-5.92)		 3.41
(2.57-6.54)		 3.43
(2.71-6.99)		 4.44
(3.15-6.54)		 4.66
(3.11-6.82)		 3.46
(2.71-4.47)		 3.55
(2.86-4.68)
Bias
(95%CI)		 3.38 (-0.20 to 6.97) 2.83 (0.71 to 4.96) 2.37 (-1.27 to 6.01) 2.23 (-1.99 to 6.46) 4.00 (0.17 to 7.84) ± 6.9 2.49 14.00 39.42
Lym
(x109 /L)		 2.08
(1.75-2.36)		 2.20
(1.81-2.41)		 2.28
(2.01-2.98)		 2.27
(2.01-3.00)		 2.09
(1.51-2.29)		 2.24
(1.62-2.54)		 2.19
(2.01-2.39)		 2.33
(2.02-2.63)		 1.77
(1.43-2.76)		 2.17
(1.57-2.92)
Bias
(95%CI)		 3.48 (-3.00 to 9.95) 2.61 (-0.73 to 5.96) 7.26 (2.84 to 11.68) 7.42 (1.45 to 9.08) 11.60
(5.57 to 17.62)		 ± 6.3 2.90 10.80 31.00
Mon
(x109 /L)		 0.64
(0.45-0.68)		 0.62
(0.44-0.73)		 0.60
(0.50-0.68)		 0.56
(0.46-0.67)		 0.52
(0.44-0.67)		 0.51
(0.43-0.66)		 0.60
(0.51-0.68)		 0.58
(0.55-0.69)		 0.53
(0.41-0.57)		 0.50
(0.42-0.58)
Bias
(95%CI)		 1.83 (-6.08 to 9.73) -4.27 (-11.05 to 2.49) -1.80 (-9.1 to 5.51) 2.31 (-6.47 to 11.08) -0.84 (-7.57 to 5.88) ± 6.5 6.85 13.30 41.47
Eos
(x109 /L)		 0.25
(0.11-0.47)		 0.26
(0.11-0.43)		 0.27
(0.17-0.47)		 0.25
(0.20-0.49)		 0.24,
(0.11-0.38)		 0.24
(0.09-0.38)		 0.16
(0.09-0.32)		 0.14
(0.08-0.34)		 0.22
(0.06-0.33)		 0.18
(0.04-0.33)
Bias
(95%CI)		 -2.63 (-12.49 to 7.23) 1.99 (-5.20 to 9.18) -3.81 (-14.35 to 6.72) -3.00 (-13.00 to 7.01) -1.03 (-29.11 to 27.04) ± 16.8 9.48 15.00 49.19
RBC
(x 1012/L)		 4.78
(4.48-5.04)		 4.90
(4.54-5.08)		 4.78
(4.23-5.12)		 4.87
(4.29-5.16)		 4.84
(4.63-5.17)		 4.85
(4.69-5.21)		 4.90
(3.96-5.27)		 4.90
(3.95-5.21)		 4.74
(4.46-5.07)		 4.50
(4.30-4.85)
Bias
(95%CI)		 1.08 (0.15 to 2.01) 0.81(-0.22 to 1.84) 0.90(-1.33 to 3.12) -1.11 (-2.74 to 0.53) -4.07 (-5.52 to -2.62) ± 1.8 0.69 2.60 7.46
Hb
(g/L)		 128
(119-146)		 129
(122-143)		 135
(118-149)		 136
(119-148)		 142
(127-153)		 141
(129-155)		 142
(120-158)		 143
(123-158)		 133
(121-149)		 137
(122-145)		 ± 1.6 0.65 2.70 7.70
Bias
(95%CI)		 1.23 (-0.64 to 3.11) 0.97 (0.12 to 1.82) 1.38 (-1.41 to 5.38) 1.39 (-0.59 to 3.37) 0.41 (-2.57 to 3.39)
HCT
(L/L)		 0.40
(0.37-0.44)		 0.41
(0.37-0.44)		 0.41
(0.37-0.46)		 0.42
(0.37-0.46)		 0.43
(0.40-0.47)		 0.44
(0.40-0.47)		 0.43
(0.37-0.48)		 0.43
(0.37-0.47)		 0.42
(0.38-0.46)		 0.40
(0.37-0.43)
Bias
(95%CI)		 0.96 (-0.04 to 1.96) 0.49 (-0.61 to 1.60) 1.41 (-1.56 to 4.38) -1.32 (-3.03 to 0.39) -4.56 (-6.13 to -3.00) ± 1.5 0.85 2.80 8.11
Parameter Group 1
(N = 10) 		Group 2
(N = 17) 		Group 3
(N = 16) 		Group 4
(N = 7) 		Group 5
(N = 9) 		APS, %
Haemolysis 0 1 0 2 0 3 0 4 0 6 Bias CVA CVG RCV
MCV
(fL)		 87.70
(82.80-89.10)		 87.60
(83.05-89.05)		 88.50
(86.20-90.15)		 88.50
(86.00-89.75)		 90.60
(86.20-92.85)		 90.40
(86.75-92.45)		 89.10
(86.75-92.60)		 89.00
(86.75-92.40)		 90.55
(84.18-94.48)		 90.05
(84.20-93.53)
Bias
(95%CI)		 -0.07 (-0.43 to 0.29) -0.31 (-0.47 to -0.15) -0.20 (-0.60 to 0.20) -0.21(-0.55 to 0.14) -0.48 (-0.99 to -0.03) ± 0.9 0.38 0.80 2.45
MCH
(pg)		 28.30
(26.70-29.35)		 28.00
(26.95-28.70)		 28.50
(27.60-29.65)		 28.50
(27.70-29.60)		 29.30
(27.70-30.05)		 29.50
(28.35-30.20)		 29.70
(27.95-30.30)		 30.20
(29.15-30.85)		 29.05
(27.23-29.68)		 30.85
(27.85-31.05)
Bias
(95%CI)		 0.06 (-1.40 to 1.53) 0.16 (-0.65 to 0.98) 1.29 (-0.59 to 3.18) 2.59 (1.37 to 3.80) 4.65 (1.29 to 8.01) ± 1.35 1.01 0.80 3.57
MCHC
(g/L)		 322
(318-326)		 320
(319-326)		 322
(318-326)		 324
(319-329)		 324
(319-329)		 327
(319-332)		 324
(319-334)		 334
(330-341)		 321
(307-325)		 332
(326-343)
Bias
(95%CI)		 0.14 (-1.24 to 1.52) 0.47 (-0.4 to 1.33) 0.73 (-0.13 to 1.58) 2.81 (1.61 to 4.03) 5.24 (1.97 to 8.50) ± 0.4 1.10 1.00 4.12
RDW
(%)		 13.10
(11.85-15.00)		 13.10
(11.95-15.10)		 12.90
(12.35-13.05)		 12.80
(12.40-13.10)		 12.90
(12.45-14.45)		 12.80
(12.45-14.25)		 12.90
(12.25-13.35)		 12.90
(12.25-13.35)		 12.70
(12.08-14.08)		 12.65
(12.23-14.15)
Bias
(95%CI)		 0.63 (0.01 to 1.25) 0.12 (-0.20 to 0.45) 0.15 (-0.44 to 0.74) 0.11 (-0.43 to 0.65) 0.26 (-0.60 to 1.12) ± 1.2 0.53 1.70 4.94
Platelet count
(x109/L)		 251
(182-252)		 249
(179-292)		 251
(214-275)		 250
(217-274)		 245
(213-284)		 255
(216-295)		 261
(231-302)		 262
(237-303)		 257
(200-301)		 260
(207-319)
Bias
(95%CI)		 -0.11 (-2.30 to 2.07) -0.53 (-1.5to 0.43) 1.34 (-0.75 to 3.44) 0.63 (-2.22 to 3.49) 3.21 (0.98 to 5.44) ± 5.0 1.74 5.60 16.25
MPV
(fL)		 9.53
(7.48-10.75)		 9.18
(7.50-10.65)		 8.84
(8.08-10.16)		 8.76
(8.07-10.04)		 8.00
(7.62-9.64)		 7.95
(7.54-9.64)		 8.16
(7.19-8.63)		 8.20
(7.22-8.57)		 9.63
(8.32-10.73)		 9.45
(8.45-10.65)
Bias
(95%CI)		 -0.49 (-1.83 to 0.84) -0.1 (-0.96 to 0.76) -0.09 (-0.72 to 0.91) -0.29 (-1.56 to 0.97) -0.47 (-2.02 to 1.07) ± 1.9 0.62 2.30 6.60
Data are presented as median (interquartile range). APS - analytical performance specifications. CVA - analytical variation. CVG - within-subject biological variation. RCV - reference change value. WBC - white blood cell. Neu - neutrophil. Lym - lymphocyte. Mono - monocyte. Eos - eosinophil. Hb – haemoglobin. RBC - red blood cells. HCT – haematocrit. MCV - mean corpuscular volume. MCH - mean corpuscular haemoglobin. MCHC - mean corpuscular haemoglobin concentration. RDW - red cell distribution width. PLT – platelet. MPV - mean platelet volume. CI - confidence interval.