Figure 5.
LFIA analysis of the extracted corn sample spiked with AFB1 standard using latex microsphere-sAFH-3e3 IgG antibody. The corn sample was extracted with PBS buffer or 70% (v/v) methanol and spiked with various concentrations of AFB1. The green color in the test line (T) decreased when the concentration of AFB1 was increased. The graph was plotted from A/A0 against log of concentration of aflatoxin; A = peak area of each concentration of spiked AFB1 and A0 = peak area at 0 ppb.