Table 4. Primers for the Construction scFv-Fc and IgG Expression Vectors Used in This Studya.
| primer | sequence |
|---|---|
| Fc-BspEIFw | 5′AGT CTC CGG AGA GCC CAA GAG CTG CGA C3′ |
| CH-NheIFw | 5′ATG CGC TAG CAC CAA GGG CCC CAG CGT GTT CC3′ |
| CH-AgeIRv | 5′GCC ACC GGT TCA CTT GCC GGG GGA CAG GCT3′ |
| C3 VLNheIFw | 5′AGT GCC GCT AGC GAC ACC GTG ATG ACC CAG TCT3′ |
| LC conAgeIRv | 5′AGT GCC ACC GGT CTA ACA CTC TCC CCT GTT3′ |
| C3 VHNheIFw | 5′GCC GCT AGC CAG GTG CAG CTG GTG CAG TC3′ |
| 3E3 VHNheIFw | 5′AGT GCC GCT AGC GGG GTG CAG CTG GTG GAG TC3′ |
| VL BspEIRv | 5′GCA CAG TCC GGA ACG TTT GAT CTC CAC CTT GGT3′ |
| C3 VHNheIRv | 5′TTG GTG CTA GCT GAG GAG ACG GTG ACC AGG G3′ |
| C3 VLNheIFw | 5′AGT GCC GCT AGC GAC ACC GTG ATG ACC CAG TCT3′ |
a
Sequences corresponding to NheI, BspEI, BsiWI, and AgeI restriction sites are underlined.