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. 2021 Aug 30;297(4):101148. doi: 10.1016/j.jbc.2021.101148

Figure 1.

Figure 1

The MRN complex is prematurely disassembled from double-strand break sites in RecQL4-defective cells.A and B, immunostaining of the MRN component proteins. U2OS cells transfected with RecQL4 or GL2 (siGL) siRNAs were treated with neocarzinostatin for 15 min and incubated in a fresh medium for the indicated times. Representative images of Mre11 immunostaining (A) and graphs for the percentages of foci-positive cells (B) are shown. Data in graphs are means ± SD; n = 3. The scale bar represents 10 μm. C, Western blots of U2OS cells prepared as in (A) and (B). D, EGFP-Mre11 binding to microirradiation sites in RecQL4- (siR4) or mock-depleted (siGL) U2OS cells. Data in graphs are means ± SD; n = 25. The scale bar represents 10 μm. E, immunostaining of Mre11 in Rothmund–Thomson syndrome (RTS) cells (AG17524) transfected with empty (EV) or RecQL4 expression vectors. Cells were treated with neocarzinostatin for 15 min and incubated in a fresh medium for the indicated times. The scale bar represents 10 μm.