Skip to main content
. 2021 Aug 30;297(4):101148. doi: 10.1016/j.jbc.2021.101148

Figure 2.

Figure 2

Stable maintenance of the MRN complex on double-strand break sites requires helicase activity of RecQL4.A, immunostaining of RecQL4-depleted U2OS cells transfected with wildtype (WT), Walker A (WA), or Walker B (WB) mutant RecQL4. Cells were treated with neocarzinostatin and then incubated in fresh medium for the indicated times. Right panel is the Western blot showing depletion of endogenous RecQL4 and expression of wildtype and mutant RecQL4 proteins. B, immunostaining of Mre11 and Cyclin A in mock- (siGL) or RecQL4-depleted U2OS cells treated with neocarzinostatin and released for 0 or 1 h. The scale bar represents 10 μm.