Figure 5.

Stress-induced instability facilitates adaptation through stress and locus-specific copy number changes. (A) Synergistic effect of transcription and Hydroxyurea (HU) induced replication stress on instability at (i) rDNA and (ii) CUP1 arrays. Error bars represent standard deviation based on four biological replicates. Statistical significance was calculated using a standard two-tailed t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. (B) The wild-type CUP1-MATα strain (2×CUP1) was subcultured in the indicated medium for ∼6 days (approximately 50 generations). After every 3 days, three independent isolates were used to measure CUP1 copy number by ddPCR. Error bars represent standard deviation for each individual reaction.