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H3K56 acetylation restricts transcription-induced amplification of the CUP1 array. (i) Wild-type, (ii) rtt109Δ or (iii) hst3Δhst4Δ CUP1-MATα strains (6×CUP1) were subcultured in the indicated medium for ∼6 days (approximately 50 generations). After every 3 days, three independent isolates were used to measure CUP1 copy number by ddPCR. Error bars represent standard deviation for each individual reaction.
