. 2021 Aug 31;11(19):13348–13362. doi: 10.1002/ece3.8057

TABLE 2.

Barcode resolution based on BLAST searches, using the generated DNA barcode library as both query and local database, and phylogenetic resolution of rbcL and ITS2, considering only nodes with bootstrap support (BS) ≥ 70%

Marker	IS ^a	NIS ^b	NA ^c	%IS ^d	%SS ^e	SSA ^f	SMA ^g	MS ^h	NMS ⁱ	%SMA ^j	%MS ^k
rbcL	369	123	46	75.00	91.45	309	183	114	69	37.20	62.30
rbcL_reduced ^l	319	74	145	81.17	73.05	259	134	91	43	34.10	67.91
ITS2	390	46	102	89.45	81.04	283	153	115	38	35.09	75.16
ITS2_reduced ^l	351	42	145	89.31	73.05	259	134	102	32	34.10	76.12
rbcL + ITS2	463	67	8	86.06	98.51	324	206	136	70	38.87	66.02
rbcL + ITS2_reduced ^l	356	37	145	90.59	73.05	259	134	107	27	34.10	79.85

The analyses included specimens of all 543 species of the canga of Serra dos Carajás and other regions in the Brazilian state of Pará, Eastern Amazon (Table A1). The detailed data on the barcode resolution (IS ^a ) and phylogenetic resolution (MS ^h ) of each species are available in the Supplementary Table S3 (https://osf.io/5xt3u/).

^{^a}

IS, number of correctly identified species.

^{^b}

NIS, number of nonidentified species.

^{^c}

NA number of species with no available sequences.

^{^d}

%IS, percentage of correctly identified species.

^{^e}

%SS, percentage of species with available sequences.

^{^f}

SSA, number of species with a single accession with available sequences.

^{^g}

SMA, number of species with more than one accession with available sequences.

^{^h}

MS, number of species recovered as monophyletic, with BS ≥ 70%.

^ⁱ

NMS, number of species recovered either as nonmonophyletic or with BS > 70%.

^{^j}

%SMA, percentage of species with more than one accession.

^{^k}

%MS, percentage of species recovered as monophyletic, with BS ≥ 70%.

^{^l}

Including only the species with sequences of both rbcL and ITS2.