C. lusitaniae isolates with low activity Mrr1 variants, U05 (green) and L14 (blue), or with constitutively active Mrr1 variants, L17 (grey) and S18 (dark red), were grown at 37°C in YPD with FLZ in the presence or absence of 5 mM MG. (A) Growth kinetics of isolates U05, L14, S18, and L17 in FLZ with (open circles) or without (closed circles) 5 mM MG. Data shown represent the mean ± SD from three independent experiments. (B) OD600 after 16 hours of growth for each indicated strain at 37°C in FLZ with or without 5 mM MG as indicated. Data shown represent the mean ± SD from three independent experiments. Ordinary two-way ANOVA Tukey’s multiple comparison test was used for statistical analysis; a-b, p < 0.05.