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. 2021 Apr 19;11(7):jkab127. doi: 10.1093/g3journal/jkab127

Figure 2.

Figure 2

Mcr, Cont, Nrx-IV, and Cora localization at later stages of oogenesis. (A) Diagram of a stage 12 egg chamber. (B–I) Confocal optical sections of wild-type stages 11 and 12 egg chambers (B, D, F, and H) or stage 13 and14 egg chambers (C, E, G, and I) stained with antibodies against Mcr (B and C), Cont (D and E), Nrx-IV (F and G), or Cora (H and I) (magenta and in individual channel in B’, D’, F’, and H’) and co-stained with antibodies against Ecad (green) and labeled with DAPI (blue). The location of these sections overlaps the boundary between the oocyte (Oo.) and NCs and is depicted as the red box in the diagram shown in (A). Note that Mcr, Cont, Nrx-IV, and Cora become enriched at the apical-lateral region of FCs membrane (arrows in B, D, F, and H). Insets show higher magnification of the indicated areas. The expression of all of these proteins persists in stage 13/14 egg chambers (C, E, G, and I). Anterior is to the left. Scale bar = 25 µm in B, D, F, and H and 100 µm in C, E, G, and I.