Fig. 9. PrOF NMR studies characterizing binding site interactions for SPSB2 and AMA1. (A) SPSB2 (grey) bound to DINNN (orange) with tryptophan residues in green (PDB: 3EMW, top); ¹⁹F NMR spectra of 5FW-SPSB2 alone, bound to a DINNN peptide, and bound to peptidomimetics M1, M2, M3, M4 (bottom, adapted from ref. 21 with permission from MDPI, copyright 2016). (B) AMA1 in apo form (grey) highlighting the DII loop in blue and tryptophan residues in green, both mutated and native (PDB: 2Z8V, top); ¹⁹F NMR spectra of F367W 5FW-AMA1 alone, bound to peptides R1 and RON2L, and bound to a series of aminothiazole fragments (0,1,3 μM, bottom, adapted from ref. 126 with permission from Royal Society of Chemistry, copyright 2016).