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. 2021 Oct 7;12:523. doi: 10.1186/s13287-021-02597-y

Fig. 3.

Fig. 3

Actin filaments regulated early stage of osteogenic differentiation induced by CTS via integrin αVβ3 activation in fibroblasts. a Protein expression of β-actin under CTS for 4 d and 7 d. b mRNA expression of β-actin under CTS for 4 d and 7 d. c Protein expression of β-actin under CTS in the GM and RGDyk groups. d mRNA expression of β-actin under CTS in the GM and RGDyk groups. e Protein expression of β-actin under CTS in the GM, NC, shRNA-integrin αV and shRNA-integrin β3 groups. f mRNA expression of β-actin under CTS in the GM, NC, shRNA-integrin αV and shRNA-integrin β3 groups. g Protein expression of β-actin under CTS in the CTS and Cyto D groups. h Immunofluorescence of F-actin under CTS in the GM, CTS, NC, shRNA-integrin αV and shRNA-integrin β3 groups for 7 d. Scale bar, 50 μm